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Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.
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Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

doi: 10.1073/pnas.2521091122

Figure Lengend Snippet: Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

Techniques:

Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

doi: 10.1073/pnas.2521091122

Figure Lengend Snippet: Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

Techniques: